flotation cell fusion

flotation assay to detect changes in the hydrophobicity of virions, and a protease susceptibility assay to identify the domain of the viral spike protein that undergoes conformational change. ... with increased cell fusion activity of S (Stunnan and Holmes, 1977; Frana, et aI., 1985)

Jun 10, 2019 Jun 10, 2019 Cell-cell fusion. This three-color fluorescence assay was performed as previously described (Cifuentes-Mu oz et al., 2011). Vero E6 cells (ATCC) seeded into 16 well chamber slides were transfected with the pI.18/GPC wild type or the different mutant

tion on the top of the flotation solution. Centrifuge the tube at 50g for 1. 0. ... The technique of cell fusion has already been applied to produce a large number of intergeneric, intertribal, or

Jul 19, 2021 Recognition and fusion between gametes during fertilization is an ancient process. Protein HAP2, recognized as the primordial eukaryotic gamete fusogen, is a structural homolog of viral class II

Nov 02, 2003 Nov 02, 2003 Cells were incubated in fusion buffer 21 at the indicated pH for 10 min at 37 C and then reneutralized with fusion buffer, pH 7.0. Cells were then lysed as described above. Cells

Jun 01, 2017 The two-factor fusion system described here opens the door to programmable and potentially targeted cell fusion, which may find therapeutic and research application in oncolytic fusion of cancer

J. Cell Sci. ii, 59-75 (1972) Printed in Great Britain FINE-STRUCTURAL STUDIES ON SPONTANEOUS AND INDUCED FUSION OF HIGHER PLANT PROTOPLASTS LYNDSEY A. WITHER S AND E. C. COCKING Department of Botany, University Park, Nottingham NG7 zRD, England SUMMARY During the treatment of some plant tissues with cell-wall-degrading enzymes adjacent cells

Sep 15, 2021 HCV entry involves cell-type recognition and binding, translocation to the tight junctions and membrane fusion to the endosome. HCV fusion requires both E1 and E2 glycoproteins as well as low pH

Nov 27, 2012 Glycoprotein E (gE) of HSV plays a key role in cell-to-cell spread and virus-induced cell fusion. Here, we report that this function of gE requires the cooperation of tegument proteins UL11, UL16, and UL21. We found that the four proteins come together with very high efficiency to form a complex in transfected cells and in a manner that is regulated and coordinated

To confirm that β-galactosidase/706-856, which floated to fractions 6 and 7, was membrane associated, the postnuclear fraction obtained from cells expressing the fusion protein was either treated with 1% SDS or left untreated prior to membrane flotation assay

Jun 11, 2015 Jun 11, 2015 Protoplast fusion 1. PROTOPLAST FUSION Presented by- Neetu Nand Ph.D 1st Semester 2. INTRODUCTION •A protoplast is a plant, bacterial or fungal cell that had its cell wall completely or partially removed using either mechanical or enzymatic means. Protoplasts: Have their cell wall entirely removed Spheroplasts: Have their cell wall only partially removed • More generally protoplast refers

Jun 01, 2017 Figure 7 | Minion and Myomaker are sufficient to induce fusion of non-muscle cells. ( a) W estern blot of 10T1/2 fibroblasts expressing Luciferase (Ctrl), Myomaker or Minion. n 2. ( b

Apr 16, 2012 ER–ER fusion is delayed in cells missing Sey1p. (a) Wild-type cells of opposite mating types expressing either ss-RFP-HDEL or cytosolic GFP were mixed, placed on an agarose pad, and imaged at 1-min intervals. Selected images from the time-lapse video are shown. Time 0 is the first image taken after cell fusion, as indicated by GFP in both cells

After solubilization of cells expressing the F protein with 1% Triton X-100 at 4 degrees C, ca. 40% of total, cell-associated F protein fractionated with classical DRMs with densities of 1.07 to l.14 as defined by flotation into sucrose density gradients

tion on the top of the flotation solution. Centrifuge the tube at 50g for 1. 0. ... The technique of cell fusion has already been applied to produce a large number of intergeneric, intertribal, or

Jun 01, 2020 Jun 01, 2020 This observation led to the test for cell–cell fusion without the viral background using transfected fibroblasts and therefore defined the minimal set of HSV-1 proteins involved in membrane fusion as gD, gB and gH/L being both necessary and sufficient to mediate plasma membrane fusion . As those proteins do not share any structural similarity, the theory is that during fusion, each protein is responsible for a distinct aspect of the process, together forming a multicomponent fusion

Jun 10, 2019 Jun 10, 2019 When we assayed the pH for triggering cell-cell fusion by the E26Q mutant we observed an activation pH of 6.2, that is 0.2 units higher than wild type . Such a raise in the activation pH may be explained by the loss of a salt bridge between Glu26 and Arg301 in the Gc:Gc inter-spike dimer ( Figure 1b ), facilitating spike dissociation and therefore leading to fusion at a less acidic pH compared to wild type

J. Cell Sci. ii, 59-75 (1972) Printed in Great Britain FINE-STRUCTURAL STUDIES ON SPONTANEOUS AND INDUCED FUSION OF HIGHER PLANT PROTOPLASTS LYNDSEY A. WITHER S AND E. C. COCKING Department of Botany, University Park, Nottingham NG7 zRD, England SUMMARY During the treatment of some plant tissues with cell-wall-degrading enzymes adjacent cells